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STC-1 cells, an enteroendocrine intestinal cell line, were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 15% (v/v) horse serum, 2.5% (v/v) fetal bovine serum (FBS; Zhejiang Tianhang Biological Technology Co., Ltd., Huzhou, China), and 25 mmol/L glucose at 37 °C in a 5% CO2 humidified atmosphere. The cells were grown to 70–80% confluence for the experiments.
Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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