2.3. Generation of Stable ZIKV-NLS-GFP Reporter Cell Lines

Lentiviral particles expressing the ZIKV-NLS-GFP reporter were generated by harvesting supernatant 72 h post transfection of 293T cells with pZIKV-NLS-GFP and the packaging plasmids psPAX2 and pMD2.G (provided by Duke Functional Genomics Facility). This supernatant was then used to transduce A549 cells for 48 h. Following transduction, cells were selected in 2 μg/mL puromycin for 48 h and then serially diluted to obtain single cell GFP-positive colonies. These clones were maintained in cDMEM containing 1 μg/mL puromycin.