Transwell migration assay

The assay was performed in a 24-well Boyden chamber with 8-μm pore size (Corning, Shanghai, China), and 4T1 cells (1 × 10⁵) were seeded in the upper chamber with TWS119 for 24 h and LY294002 for 16 h, we fixed the undersurface cells of transwell chambers with methyl alcohol and stained them with 0.1% crystal violet. A Nikon Eclipse 80i microscope and Nikon Elements Imaging System Software were used to capture images of migrated 4T1 cells.