Quantitative analysis

After image acquisition the distribution of the OB cells after either postnatal or in utero electroporation was analyzed with ImageJ software. First, images were converted to 8-bits and to facilitate the analyses of both the cell's morphology and layer localization, ICA Lut was applied. Cells were counted using the Cell Counter plug-in analysis tool of the Image J software, analyzing four P25-30 mice animals per group (IUE or postnatal electroporation). Up to 1875 OB cells were counted following postnatal electroporation and 926 cells from the in utero electroporated animals. The somata area of DCX positive cells was analyzed with the ImageJ software (60 cells within the granular cell layer and 40 within the subependymal zone). Statistical analyses were performed with SigmaPlot 13 (Systat Software) and the values were represented as the mean ± SEM. T-tests were performed to determine the significance between different groups, or with the Mann-Whitney Rank Sum Test when the normality test failed. A confidence interval of 95% (p < 0.05) was required to considered values statistically significant.