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The siRNA oligonucleotides corresponding to human MYCN, FH, and Nrf2 were purchased from Fisher Scientific Inc. siRNA oligonucleotides (20 nM) were transfected into cells using Lipofectamine RNAiMAX reagent (Invitrogen). After 48–72 h of transfection, IBs were carried out to examine the knockdown of targeted proteins.
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