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2.14. Cellular Thermal Shift Assay (CETSA)
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PC12 cells were collected and freeze-thawed five times using liquid nitrogen. The cell lysates were grouped into two aliquots, with one serving as the control and the other being treated with ICA (20 μM) for 4 h at 4°C. Then, the lysates were heated at indicated temperatures (48 and 66°C, respectively) for 5 min followed by cooling at room temperature. The protein bans were detected by immunoblot [24, 26].
Copyright and License information: ©2019 Shao-Yang Zhao et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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