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Molecular detection of carbapenemase genes using multiplex-PCR
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All K. pneumoniae isolates were tested for common carbapenemase genes (blaIMP, blaOXA-48, blaVIM, blaNDM, and blaKPC). Multiplex-PCR was performed according to the protocol of Zarakolu et al10 using a series of primers (Table 1) that were obtained from Macrogen (Seoul, South Korea).
Primers used in this study.
Copyright and License information: ©2018 Saudi Medical Journal
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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