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Metabolic stability assay.
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The stability of test compounds were determined by incubation with liver microsomes derived from rat, mouse or human. All assays were conducted by Absorption Systems (Exton, PA) by methods previously described (Clarke and Jeffrey, 2001. Test compounds at 1 μΜ were assayed at various times of incubation up to 60 min. Samples were analyzed using a LTQ-Orbitrap XL mass spectrometer. The assay was a single determination at each time point. As a metabolically labile control, 1 μΜ testosterone was tested.
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