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Hydrodynamic tail vein injection

JW Jingxiao Wang
MD Mingjie Dong
ZX Zhong Xu
XS Xinhua Song
SZ Shanshan Zhang
YQ Yu Qiao
LC Li Che
JG John Gordan
KH Kaiwen Hu
YL Yan Liu
DC Diego F. Calvisi
XC Xin Chen
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We employed hydrodynamic injection as previously described in detail [47]. The dosage of pT3-EF1α-HA-myr-AKT (AKT) and pT3-EF1α-YapS127A (Yap) plasmids was 20 and 30 μg, respectively. To suppress the canonical Notch signaling, we applied 60 μg pT3-EF1α-dnRBPJ or 60 μg pT3-EF1α together with AKT and Yap plasmids in FVB/N mice. In transgenic models, we injected 60 μg pCMV-Cre or 60 μg empty pCMV vector together with AKT/Yap. Mice were housed, fed, and monitored according to protocols approved by the Committee for Animal Research at the University of California San Francisco (San Francisco, CA).

Copyright and License information: The Author(s) ©2018
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