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10 µm magnetic beads (Millipore) were coupled with 100 µg ml−1 biotinylated anti-PSMA antibody (clone LNI-17, BioLegend) for 1hour at room temperature with gentle agitation. EV capture was performed overnight at room temperature with rotation. For staining, EV-coated beads were blocked with Fc Block in a saline buffer containing 5 mg ml−1 casein, 25 mM Tris and 150 mM NaCl at pH7.4, and then PE-conjugated anti-PSMA was added at 10 µg ml−1 in same buffer for 15 min.
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