2.2. Obtaining Extracts from Macroalgae and Microalgae by UAE Method

Each of the algae (5 g) was extracted using a mixture consisting of water/ethanol (50:50, v:v) (50 mL) in glass bottles. The extraction was performed in an ultrasonic bath (Branson ultrasonic M3800-E, Dietzenbach, Germany) at room temperature for 30 min. After extraction, the solvent was separated from the alga by centrifuging at 3000× g for 10 min at 4 °C and filtering with filter paper using a vacuum to remove the algal material. The supernatant was stored at −20 °C until analysis. Extraction yield, total phenolic content (TPC) and antioxidant activity (ORAC, ABTS, DPPH and FRAP assays) were evaluated for the obtained extracts, as described below. All experiments were performed in duplicate.