Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Peripheral blood mononuclear cells (PBMCs) were isolated within 24 hours after blood withdrawal, cultured in X‐Vivo (48 hours) unstimulated (U), stimulated with plate‐bound anti‐CD3 (3 μg/mL) plus soluble anti‐CD28 (1 μg/mL), lipid A (LpA, 0.1 μg/mL), or peptidoglycan (PGN, 1 mg/mL, OR) at 37°C. Cell pellets were used for RNA isolation utilizing the RNeasy Mini Kit (Qiagen, Hilden, Germany), and supernatants were frozen at −80°C. Genomic DNA was extracted from whole blood (Flexigene DNA‐Kit, Qiagen).
Copyright and License information: ©2019 The Authors. Published by John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
