Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Porcine cumulus-oocyte complexes (COCs) were collected from the follicles (3-6 mm diameter) and cultured in 500 μL droplets of in vitro maturation (IVM) medium overlaid with paraffin oil at 39℃, 5% CO2 in air for 42-44 h. The IVM medium was Tissue Culture Medium 199 (TCM199; Gibco) supplemented with 0.1% polyvinyl alcohol (PVA), 3.05 mM D-glucose, 0.91 mM Na-pyruvate, 75 μg/mL penicillin G, 50 μg/mL streptomycin, 0.57 mM cysteine, 10 ng/mL epidermal growth factor, 0.5 μg/mL luteinizing hormone, and 0.5 μg/mL follicle stimulating hormone.
Copyright and License information: ©2018 The Korean Society of Developmental
Biology
This is an Open-Access article distributed under the terms of the
Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any
medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
