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For transient knockdown experiments, the small-interfering RNAs (siRNAs) were as follows: FEZF1-AS1 siRNA (si-FEZF1-AS1), ZNF312B siRNA (si-ZNF312B) and synthetic sequence-scrambled siRNA (si-NC) were purchased from GenePharma Co. (Shanghai, China). Stable suppression of FEZF1-AS1 was performed by short-hairpin RNA (sh-RNA) interference. All oligonucleotide sequences are listed in Table S2 (see Additional file 2). The details of transfection and infection procedures are described in Supplementary Methods.
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