siRNA transfection

TK Toshiyuki Kobayashi
AS Atsushi Shiozaki
YN Yoshito Nako
DI Daisuke Ichikawa
TK Toshiyuki Kosuga
KS Katsutoshi Shoda
TA Tomohiro Arita
HK Hirotaka Konishi
SK Shuhei Komatsu
TK Takeshi Kubota
HF Hitoshi Fujiwara
KO Kazuma Okamoto
MK Mitsuo Kishimoto
EK Eiichi Konishi
YM Yoshinori Marunaka
EO Eigo Otsuji
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Cells were transfected with 12 nmol/L CLIC1 siRNA (Stealth RNAi siRNA #HSS101987, Invitrogen, Carlsbad, CA, USA) using the Lipofectamine RNAiMAX reagent (Invitrogen), according to the manufacturer’s instructions. Medium containing siRNA was replaced with fresh medium after 24 h. Control siRNA (Stealth RNAi siRNA Negative Control; Invitrogen) was used as a negative control.

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