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4.4. Histological Observation
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After treatments, all rats were sacrificed simultaneously. Approximately 6 cm of the colon lesion was reserved at 6-8 cm distance from the anus. A 1-cm length of the dissected colon was removed, washed with iced saline, fixed in 10 % formalin, embedded in paraffin, sectioned, stained with hematoxylin and eosin, dehydrated in 95, 90, and 70 % ethanol, cleared in xylene, mounted in Permount or Histoclad, and observed under a light microscope.
Copyright and License information: ©2019 Yin Shi et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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