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2.5. Determination of entrapment efficiency and drug loading

MZ Meiling Zhou
JH Jierong Hou
ZZ Zhirong Zhong
NH Na Hao
YL Yan Lin
CL Chunhong Li
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Encapsulation efficiency (EE) and drug loading (DL) were measured using the sodium citrate ultracentrifugation method. The desired amount of SLNs/PD was dispersed in 10% (w/v) sodium citrate solution and centrifuged for 40 min at 40,000 rpm (Beckman-Coulter, Brea, CA) to separate free PD from SLNs/PD. The same amount of SLNs/PD was dissolved in methanol, and the amount of PD was determined by high-performance liquid chromatography (HPLC; Agilent, Santa Clara, CA) using a Kromasil ODS-1 C18 column (150 × 4.6 mm, 5 µm) and a mobile phase of acetonitrile–water.

Copyright and License information:  ©2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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