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RNA isolation and RT-PCR from paraffin sections

JH J. R. Hens
IS I. Sinha
FP F. Perodin
TC T. Cooper
RS R. Sinha
JP J. Plummer
CP C. E. Perrone
DO D. Orentreich
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Ten micron sections were obtained from the formalin-fixed, paraffin-embedded MCF10AT1 tumors and mammary fat pads of the athymic nude mice. Five sections were collected in a microcentrifuge tube, and RNA was isolated using miRCURY RNA isolation kit (Exiqon, Woburn, MA) according to the manufacturer’s instructions. RNA (250 ng) was converted into cDNA for real-time PCR analysis and analyzed as described above.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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