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The slides were deparaffinized in xylene and rehydrated in alcohol. After, PSR staining was performed using a commercial kit (HistokitTM, Easypath, SP, Brazil), according to manufacturer’s instructions. The slides were examined in an optical microscopy with polarized light (Axio Imager A1, Zeiss®, Göttingen, Germany). The collagen fibers that presented red-orange birefringence were considered type I, while the collagen fibers with green birefringence were interpreted as type III (Coll-III) [21].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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