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4.2. Alkaline Phosphatase Activity (ALP) Assay
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The activities of ALP were determined using an enzyme assay kit, according to the manufacturer’s protocol (Alkaline phosphatase assay kit, colorimetric (Abcam#83369) Abcam PLC, Cambridge, MA, USA). ALP activity was calculated as picomole/min/mL from the cells that were harvested between 0 and 4-day post-confluence.
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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