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Streptavidin-HRP staining for anti-DMPO probe

RT Rheal A. Towner
DS D. Saunders
NS N. Smith
WT W. Towler
MC M. Cruz
SD S. Do
JM J. E. Maher
KW K. Whitaker
ML M. Lerner
KM K. A. Morton
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Necropsies were performed on all rats. Brain tissues were fixed in 10% phosphate buffered formalin, embedded in paraffin, serially sectioned at 5 μm, and were stained with hematoxylin eosin (H&E) for histological examination. Streptavidin-HRP was used to target the biotin moiety of the anti-DMPO probe bound to macromolecular DMPO-trapped free radicals in excised rat brain tissues from the hippocampus region. Nova Red was used as the chromogen for HRP staining.

Copyright and License information: American Aging Association ©2018

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