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Silver in-situ hybridization (SISH)

LM Lisa Mäder
AB Anna E. Blank
DC David Capper
JJ Janina Jansong
PB Peter Baumgarten
NW Naita M. Wirsik
CZ Cornelia Zachskorn
JE Jakob Ehlers
MS Michael Seifert
BK Barbara Klink
SL Stefan Liebner
SN Simone Niclou
UN Ulrike Naumann
PH Patrick N. Harter
MM Michel Mittelbronn
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SISH of Epidermal growth factor receptor (EGFR)-amplification was performed using the BenchMark XT IHC/ISH system (Ventana). After deparaffinization and pretreatment, slides were incubated with one drop of ISH-Protease followed by the EGFR probe. The visualization was performed with Silver C. A chromosome 7 probe (Red ISH V probe) was used as control. Afterwards the sections were washed, counterstained with hematotoxylin and bluing reagent and mounted.

Copyright and License information:  ©2018 Mäder et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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