Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Cell cycle analysis

VK Vivek K. Kashyap
QW Qinghui Wang
SS Saini Setua
PN Prashanth K. B. Nagesh
NC Neeraj Chauhan
SK Sonam Kumari
PC Pallabita Chowdhury
DM Duane D. Miller
MY Murali M. Yallapu
WL Wei Li
MJ Meena Jaggi
BH Bilal Bin Hafeez
SC Subhash C. Chauhan
ask Ask a question
Favorite

Effect of VERU-111 on cell cycle arrest of PanCa (Panc-1 and AsPC-1) cells was analyzed by flow cytometric analysis. Briefly, approximately 70% confluent cells were synchronized by overnight incubation in FBS free media. Cells were treated with VERU-111 (0, 5, 10, and 20 nM) for 24 h, harvested, fixed overnight in ice-cold ethanol (70%) followed by incubation with RNAse and incubated with the DNA staining Propidium iodide (Sigma) for flow cytometry. Data regarding the number of cells in different phases of the cell cycle was analyzed by BD Accuri C6; Becton-Dickinson, Mountain View, CA.

Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A