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Cell proliferation was determined using a cell counting kit‐8 (CCK‐8) assay according to the manufacturer's protocol. Briefly, cells were seeded into 96‐well plates at 6 × 103 cells/well. An aliquot of 10 μL CCK‐8 solution was added to each well, and the plate was incubated for 4 h at 37°C. At the indicated time points, the absorbance at 450 nm was measured using a spectrophotometer. For each group, data from five wells were pooled.
Copyright and License information: ©2018 The Authors. published by John Wiley & Sons Ltd.
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