Cell migration assay (wound healing assay)

A scratch wound assay was conducted to assess the influence of Tz and RA on cell migration. SKBR3 and BT-474 cells were seeded in 96-well plates and incubated until 80% confluence. Wounds were made in the monolayers by scratching the surface with a pipette tip (10 μl) as uniformly and straight as possible. The cells were washed and the treatments prepared in 200 μl of medium added. Cell migration was monitored for 72 h. Digital images from cells were taken with a 16X objective after 24, 48 and 72 h. The distance of migration was then analyzed by phase-contrast microscopy and closed areas were quantified using imageJ software.