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2.2. Urine Protein/Urine Creatinine Determination
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Urine protein was detected by the Coomassie Brilliant Blue method (Tiangen). Urine (1 μl), ddH2O (14 μl), and CBB staining solution (285 μl) were added to 96-well plates. Protein concentration was assessed on Thermo Scientific Microplate Reader at 595 nm. Urine creatinine was detected with Creatinine Assay Kit (Nanjing Jiancheng Bioengineering Institute), according to the manufacturer's instructions. Finally, urine protein was normalized to urine creatinine.
Copyright and License information: ©2018 Wanbing Chen et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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