Binge-like Feeding Assay

The Binge-like Assay was performed as previously described (Gaykema et al., 2014). On the night before testing, mice (N = 10) received a small (<0.1 g) sample of either a highly palatable high fat, high sugar diet (HFD; Research Diets Inc., {"type":"entrez-nucleotide","attrs":{"text":"D12331","term_id":"2148494","term_text":"D12331"}}D12331, 5.56 kcal/g) in a petri dish to familiarize them with this novel food. At lights-on all food was removed, and mice received an injection i.p. of either saline or CNO, equal volume. After 30 min mice were challenged with ~1.5 g of pre-weighed HFD in a petri dish, and allowed to consume. After 15 min, 30 min, and 1 h the food was removed and weighed, then replaced in the cage. After 1 h mice were returned to ad lib chow feeding. The experiment was repeated for 2 days total, such that each mouse received HFD + saline and HFD + CNO. Mice were assigned randomly to begin the experiment on either saline or CNO.