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Gene Expression Analysis

PP Pattarawan Pattamaprapanont
CG Christian Garde
OF Odile Fabre
RB Romain Barrès
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Total RNA was isolated from cells with TRIzol (Invitrogen) or AllPrep DNA/RNA/miRNA universal kit (Qiagen) according to the manufacturer’s instructions. RNA was converted to cDNA by iScript cDNA synthesis kit (Bio-Rad). Gene expression was determined by quantitative real-time PCR (qPCR) using Brilliant III Ultra-Fast SYBR Green Master mix (Agilent Technologies). Sequences of qPCR primers used for Ppargc1a, Ppard, Cyclin D1 (Ccnd1), and Nr4a3 genes are provided (Table S2 in Supplementary Material). mRNA expression was quantified by normalizing Ct values to cDNA amounts.

Copyright and License information:  ©2016 Pattamaprapanont, Garde, Fabre and Barrès.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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