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Data are presented as the mean ± standard error (SE). Statistical analysis was performed using SPSS 19 software and determined by a two-tailed Student’s t-test. Differences were considered significant when p < 0.05. ImageJ software was used to measure the distance between the edges of the scratches. Figures were created using GraphPad Prism version 4 software (GraphPad Software, Inc., La Jolla, CA, USA). Additionally, the dose–response curve function of this software was used to determine the IC25 and IC50 doses.

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