4.8. Cell Cycle Analysis

Cells cycle analysis was carried out as described previously [41]. Cell cycle analysis was analyzed after DFX117 treatment for 24 h. Briefly, cells were plated in culture dishes and cultured with fresh medium without FBS for 12 h. Then, cells were treated with DFX117 for 24 h and remove the supernatant, the treated cells were fixed with 70% ethanol overnight before staining with propidium iodide mixed with RNase. Keep the dying cells under dark conditions at room temperature for 30 min before being subjected to flow cytometry analysis.