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2.15. Transfection of YME1L Plasmid
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Amplified PCR fragments of YME1L from human cDNA bank were purified and inserted into the expression vector pcDNA3.1 according to the manufacturer's protocols (Gibson Assembly Mix, New England Biolabs). The constructed plasmid was propagated and sequenced to confirm the accuracy then transfected to H9C2 and detected the expression efficiency of YME1L by Western blot.
Copyright and License information: ©2018 Bangwei Wu et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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