2.13. Human protein-protein interactome between MME and INSR

The human protein-protein interaction network pulled from BioGRID (Biological General Repository for Interaction Datasets) was used to determine the shortest paths between insulin receptor (INSR) and MME. Briefly, the R package igraph was used to analyze the protein-protein interactome (PPI). The PPI (Biogrid-ALL-3.4.145) was loaded and the shortest paths between INSR and MME were determined with Djikstra's algorithm via the function all_shortest_paths. Normalized, pre-processed RNA-seq data from subcutaneous adipose tissue was taken from ENCODE [45].