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The human CRC cell lines (HCT116, H29, SW620, LOVO and SW480) and a non-tumourigenic human colorectal epithelial cell line (NCM460) were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA), streptomycin (100 μg/mL) and penicillin (100 μg/mL). Cells were maintained in a humidified atmosphere at 37 °C with 5% CO2.
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