Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

In vitro degradability and mineralization study

MR Md Shaifur Rahman
MR Md Masud Rana
LS Lucas-Sebastian Spitzhorn
NA Naznin Akhtar
MH Md Zahid Hasan
NC Naiyyum Choudhury
TF Tanja Fehm
JC Jan T. Czernuszka
JA James Adjaye
SA Sikder M. Asaduzzaman
request Request a Protocol
ask Ask a question
Favorite

To test the degradability of the scaffolds by AF-MSCs, we continued the cell attachment and growth assay for 21 days and the size-reduction of scaffold nano-powder particles was observed microscopically. After 21 days, upon degradation of the nano-powder, mineralized nodules were formed at the site of clustered AF-MSCs which were confirmed using alizarin red staining. The respective wells were washed three times with PBS and stained as described previously (Rahman et al. 2018).

Copyright and License information: The Author(s) ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A