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Coaggregation imaging

NM Naresh V. R. Mutha
WM Waleed K. Mohammed
NK Natalio Krasnogor
GT Geok Y. A. Tan
WW Wei Yee Wee
YL Yongming Li
SC Siew Woh Choo
NJ Nicholas S. Jakubovics
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Rubber ‘O’ rings of 1 mm thickness (STARLAB, Hamburg, Germany) were fixed to glass microscope slides using sticky wax or superglue. In order to keep the coaggregation stable during imaging, 0.25 volumes of agarose (1.25% wt/vol) were added to the sample. Cells were placed in the middle of the rubber ring and a coverslip (22 × 22 mm) was added. Coaggregation samples were visualized using a fluorescence microscope or a Leica SP2 CLSM microscope (Leica, Microsystems, Heidelberg, Germany) using excitation (Ex) at 530 nm and emission (Em) at 630 nm for propidium iodide and Ex/Em = 485 nm/530 nm for Picogreen.

Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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