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Measurement Of Intracellular MDA And SOD

JR JY Ren
DL DM Lin
CW CB Wang
YY YL Yang
ZW ZQ Wang
BC BQ Cui
JM J Ma
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MDA, one of the reactive products oxygen radical and unsaturated fatty acid, can be used as a maker to estimate lipid oxidative stress. When reacted with thiobarbituric acid, a chromogen which absorbs at 532 nm is formed.10 Superoxide can react with this chromogen to form a soluble formazan. SOD, one of the anti-oxidative enzyme, can inhibit the production of this water-soluble formazan.11 The intracellular ROS and MDA levels were quantified using BCA standard curve method.12 MDA was measured at 532 nm, and SOD was measured at 450 nm per protocol.13,14 Each measurement was performed six times.

Copyright and License information:  ©2019 Ren et al.
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