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Measurement Serum cf-DNA Concentrations
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Serum cf-DNA concentrations were measured using the Quant-iT PicoGreen dsDNA assay kit (Life technology). Diluted serum (1:10) was mixed with the PicoGreen dye and added to the microplate wells to make the final volume of 200 μl per well. After the microplate was incubated in the dark for 10 min, the fluorescence of the samples was measured at 480 nm excitation and 520 nm emission using BioTek Synergy2 (USA) (Zhang et al., 2014).
Copyright and License information: ©2019 Shi, Shi, Nieman, Hu, Yang, Liu, Zhu, Wei, Wu, Li, Cui and Chen.
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