MaMTH Validation Assays.

DI David M. Ichikawa
CC Carles Corbi-Verge
MS Michael J. Shen
JS Jamie Snider
VW Victoria Wong
IS Igor Stagljar
PK Philip M. Kim
MN Marcus B. Noyes
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MaMTH assays were carried out as previously described.41 In short, bait and prey plasmids were transfected into HEK293 MaMTH reporter cells, expression was induced with tetracycline, and luciferase activity was measured 24 h post induction.

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