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The effect of Q‐PAC on the demethylase activity of LSD1 was assessed in vitro using the fluorometric LSD1 Assay Kit (# 700120; Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer's instructions. Each concentration was assessed in duplicate alongside no inhibitor controls and enzyme‐only reactions, with the average fluorescence intensity from three consecutive measurements used (FLUOstar Optima; BMG Labtech (Mornington, Vic., Australia), excitation/emission 540 nm/580 nm).
Copyright and License information: ©2018 The Authors. published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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