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ChIP experiments were performed with the One Day ChIP kit (Diagenode, Seraing, Belgium). ChIP-qPCRs with gene-specific primers (Table 2) were performed using the ImmoMix PCR reagent (Bioline, Luckenwalde, Germany) in the presence of 0.1 x SYBRGreen (Molecular Probes, Thermo Fisher, Waltham, MA). Enrichment was calculated relative to input.
Copyright and License information: ©2018 Stielow et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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