ESP collection

ESP collection from the EPN was done as previously described [5]. After IJs were activated and thoroughly washed, they were transferred into a 1 L Erlenmeyer flask containing 100 mL of autoclaved PBS supplemented with 1x triple antibiotic Pen/Strep/Neo. The flask was shaken at 220 rpm in the dark for 3 hours and the nematodes were then centrifuged (700–800 rcf for 1 minute) in 15 mL conical tubes to preliminarily separate the majority of the nematodes from the PBS. The PBS supernatant was then collected and filtered through a 0.22 um syringe filter (Fisher Scientific, 9719001) and concentrated to approximately 300 μL using a 3 kD cut-off centrifuge column (Millipore, UFC900308). The protein concentration of the venom was quantified using a Bradford assay (Bio-Rad, 500–0006).