Effect of glucose metabolism inhibitors on co-metabolic degradation of β-CY by strain B-1

The energy or co-factors required by co-metabolic degradation of organic contaminants were from the microbial metabolism (Luo et al. 2014; Nzila 2013; Tran et al. 2013). Therefore, it is necessary to study the correlation between glucose metabolism and co-metabolic degradation of β-CY by strain B-1. Sodium fluoride, iodoacetic acid, trisodium phosphate and malonic acid were selected as the inhibitors of glucose metabolism (Shiota 1957; Wright 1937; Tang et al. 2011). Afterwards, a series of LB-CY media containing each of the inhibitors at 500 mg/mL were prepared. A 28.5 mL volume of LB-CY medium containing each inhibitor was mixed with 1.5 mL of inoculum in a 250 mL flask. The flask was shaken (180 rpm) at 30 °C for 72 h. The LB-CY medium without the inhibitor was used as control. Degradation of β-CY was calculated by Eq. (1), and the OD₆₀₀ was determined as previously described (Liu et al. 2014; Zhao et al. 2015).