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Transcriptional profile analysis
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For the expression profiling analysis of LRR-RLK genes, we used RNA-seq data from R. sativus tissues including flowers, siliques, leaves, stems, callus, and roots that were generated earlier and submitted to the NCBI database32,34. RNA-seq reads were aligned to the R. sativus genome using TopHat250 software. Following the alignments, we calculated transcripts abundance on the basis of fragments per kilobase of transcript per million mapped reads (FPKM) values using Cufflinks software51.
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