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Bone Marrow derived Macrophages were differentiated and used as previously described (Steele et al., 2016). BMDMs were seeded the night before infection at 200,000 cells per well in a 12 well plate. For all microscopy studies, the imaged BMDMs were seeded onto an acid treated coverslip.
Copyright and License information: ©2019, Steele et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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