2.6. Localization of MoPAS1:GFP fusion protein

Overlap cloning was performed to generate the MoPAS1:GFP vector (Han et al., 2015). A 4.4-kb fragment including a 2.4-kb ORF and 2.0-kb native promoter of MoPAS1 was amplified using GFP_F/R primers (Table 1). A 5.1-kb fragment from the plasmid pIGPAPA was amplified using pIGPAPA_F/R primers. The amplified 2.0-kb and 5.1-kb fragments were cloned using the Overlap DNA Cloning Kit (Elpis Biotech, Korea), and the fused vector was transformed into wild-type [25]. Fluorescence microscopy images of MoPAS1:GFP localization were acquired using a Carl Zeiss Axio Image A2 microscope (Carl Zeiss Microscope Division, Oberkochen, Germany).