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2.3. Variant Validations and Segregation Analysis
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Candidate variants from whole-exome sequencing were validated by Sanger sequencing. Along with this validation process, segregation analyses were performed on the family members. Primer3 v0.4.0, a web-based tool, was used to designed primers for amplification and sequencing [24]. Sequencing reactions were performed using Applied Biosystems 3130 DNA Analyzer (Life Technologies, Carlsbad, CA, USA).
Copyright and License information: ©2018 Chuphong Thongnak et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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