Trypan Blue Exclusion Assay

XS Xiaoyan Shao
XW Xueke Wang
XG Xianling Guo
KJ Ke Jiang
TY Tian Ye
JC Jianhua Chen
JF Juemin Fang
LG Linaer Gu
SW Sitong Wang
GZ Guirong Zhang
SM Songshu Meng
QX Qing Xu
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A375 and C8161 cells were infected and uninfected with NDV/FMW (MOI = 1) for 48 h, then the cell were collected and diluted in Trypan blue dye by preparing a 1:1 dilution of the cell suspension using a 0.4% Trypan blue solution for 2 min at room temperature. Cell viability was analyzed using hemocytometer. The ratio of unstained cell numbers to total cell numbers was reported as the viability percentage for each cell category.

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