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Preparation of Concentrated Supernatants
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The supernatants (6 mL) of NDV/FMW infected and uninfected cells were collected and placed into the PierceProtein Concentrator 2–6 mL/10 K filters (ThermoFisher Scientific), and then the samples were centrifugated at 1,000 rpm until the volume from 6 mL to 100 μl. The concentrated cell-free supernatants were subjected to immunoblot (IB) analysis to examine secreted HMGB1 and HSP70/90.
Copyright and License information: ©2019 Shao, Wang, Guo, Jiang, Ye, Chen, Fang, Gu, Wang, Zhang, Meng and Xu.
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