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2.5. Cycloheximide (CHX) chase assay
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Protein turnover was assessed by a standard protein‐synthesis‐inhibitor cycloheximide (CHX) chase assay. Briefly, cells were treated with 50 μg·mL−1 CHX for different times before lysis, in the presence or absence of reagents; these were then tested by western blot analysis to reveal concurrent expression levels of tested proteins.
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