2.4. Cell viability assay

The viability of NCI-H292 cells was measured using a CellTiter-Glo® luminescent cell viability assay (Promega, USA) according to the manufacturer’s protocol. Cells were seeded at a density of 1 × 104 cells/well in an opaque 96-well plate and incubated at 37 °C and 5% CO₂ for 24 h, then the cells were exposed to different concentrations of AgNPs (0, 5, 10, 25, 50, 75, and 100 µg/mL) or different concentrations of TNFα (0, 10, 20, and 40 ng/mL) for 24 h. CellTiter-Glo® reagent was added to each well and the number of viable cells was measured based on quantification of adenosine triphosphate (ATP) using a luminometer (TECAN, Japan).